Study on the relation of the characteristics of the capture sites with the Leptospira spp. occurrence in bats and rodents from Yucatan, Mexico.

This study aims to describe the natural Leptospira occurrence in small mammals from Yucatan, Mexico, and to explore the relation between the characteristics of the capture sites and the Leptospira occurrence. Bats and rodents were captured in five sites of Yucatan state, and from them, a kidney fragment was collected that was used in the genomic DNA extraction. Leptospira DNA was identified by PCR targeting the 16S-rRNA and LipL32 genes. Additionally, a bioinformatic analysis was carried out to know the Leptospira species and was corroborated with a phylogenetic tree. The assemblage of small mammals was compound of 82 (51.2 %) bats and 78 (48.8 %) rodents. A global frequency (bats plus rodents) of Leptospira occurrence of 21.2 % (34/160) was observed; in bats, it was 21.9 % (18/82), and in rodents, 20.5 % (16/78). The phylogenetic trees based on LipL32 gene showed that the recovered sequences most closely resemble the species L. borgpetersenii and L. noguchii. The ordination of the capture sites with tropical deciduous forests as original vegetation is more related to the abundance of Leptospira-infected rodents. The ordination of the capture sites with tropical sub-deciduous forests as original vegetation is more related to the diversity of Leptospira-infected bat species. The canonical ordering of the capture sites is by the original vegetation type and the diversity and abundance of Leptospira-infected bat and rodent species.

Clinical and laboratory features of rickettsioses in Yucatan, Mexico.

Rickettsia species are obligate intracellular bacteria that can cause mild to severe human disease. Based on phylogeny, clinical symptoms, and antigenic properties, rickettsiae are classified into four groups. Infections by these agents are characterized by clinical symptoms ranging from self-limited to severe and even fatal febrile illnesses, depending on the Rickettsia spp. involved, the patient's predisposition, and timely medical care. The present study aimed to characterize rickettsial diseases in Yucatan according to clinical and laboratory features appearing in medical records corresponding to 427 samples taken between 2015 and 2018. A study was conducted over the period 2015-2018 on 427 samples. Clinical and laboratory features were documented from the patients' medical records. For molecular diagnosis, blood was collected in 3.8 % sodium citrate as anticoagulant, and DNA was extracted. Single-step and nested PCR amplification was performed using genus-specific primers for the rickettsial 17kDa and ompB genes. The amplicons obtained were purified and sequenced. A total of 22.7 % (97/427) positive cases of Rickettsia spp. were identified by PCR from 14.15 % (15/106) of the municipalities in Yucatan. 75.2 % (73/97) of the cases were from the city of Merida during the autumn (September-December). The age groups with the highest frequency of confirmed cases were pediatric (5-14 years) (57.7 %) and adults (25-49 years) (42.2 %). There were six fatal cases in children, one associated with R. typhi and five with R. rickettsii. In non-fatal cases, 32.9 % (32/97) corresponded to the spotted fever group (SFG), and 60.8 % (59/97) to the typhus group (TG). Significant differences in signs, and laboratory data, were observed between the pediatric and adult populations. For the treatment of patients, oral and intravenous doxycycline was used in severe hospitalized cases. Typhus group and spotted fever group Rickettsiae are endemic pathological agents found in urban and rural areas of our region. Molecular identification allows for greater diagnostic accuracy and timely treatment and consequently a better prognosis. It is necessary to implement or reinforce measures focused on the dissemination of knowledge regarding rickettsial diseases and their prevention.

An unusual identification of Rickettsia parkeri in synanthropic rodents and domiciliated dogs of a rural community from Yucatán, Mexico.

Rickettsia parkeri belongs to the spotted fever group (SFG) of the Rickettsia genus. This bacterium causes mild rickettsiosis in humans and is mainly transmitted by Amblyomma ticks. Its medical importance is emerging in the Americas, including Mexico. Synanthropic rodents and domiciled dogs participate as accidental hosts in epidemiological cycles of Rickettsia of the SFG. The aim is to report the presence of R. parkeri in synanthropic rodents and domiciled dogs from a rural community of Yucatán, Mexico. Rodents were captured, and plasma samples were taken from dogs in 48 households from Ucú, Yucatán, Mexico. A spleen sample (rodents) and plasma (dogs) were used in the propagation of Rickettsia on Vero cells. These infected cells were used in the extraction of genomic DNA. Rickettsia DNA was identified using a semi-nested PCR (snPCR); some products were sent for sequencing. The recovered sequences were analysed with bioinformatics programs, and a phylogenetic tree was built to determine the Rickettsia species. One hundred animals were sampled: 36 synanthropic rodents and 64 dogs. The snPCR evidenced the presence of Rickettsia DNA in 10 rodents (10/36, 27.8%) and 18 dogs (18/64, 28.1%), which represents a global frequency of 28% (28/100) in this study. The bioinformatics analysis yielded homology to R. parkeri and was demonstrated in the phylogenetic tree. The first evidence of the presence of R. parkeri in synanthropic rodents (Mus musculus) from Mexico is presented; likewise, the participation of domestic dogs in the transmission cycle of this bacterium with potential importance in public health is confirmed.

Epidemiological Study of the Occurrence of Typhus Group Rickettsia Natural Infection in Domiciliated Dogs from a Rural Community in South-Eastern Mexico.

The aim is to describe the Typhus group (TG) Rickettsia infection in dogs and to identify factors associated with this infection. We collected blood samples and gathered exposure and clinical data of 142 dogs from a rural community of Yucatan. The Rickettsia group was determined by semi-nested PCR. Generalized linear models with binomial error distribution were used to model the associated factors from the dog sample for risk ratio (RR) estimation. Thirty-four dogs (23.9%) showed molecular evidence of TG Rickettsia DNA. The multivariate model showed that mixed-breed dogs (RR = 0.06) and dogs that had received antiparasitic treatment (RR = 0.049) had a lower risk of getting infected, taking as reference the purebred group and the non-treated dogs, respectively. Looking at variable interactions, adult dogs without outdoor activities had a lower infection risk than puppies (RR = 0.26). Among dogs with antiparasitic treatment, females had a higher infection risk than male dogs (RR = 26.2). The results showed enzootic TG Rickettsia circulation in dogs of a rural community. The factors outdoor activities, age and previous antiparasitic treatment, as well as the clinical variables signs of hemorrhages and epistaxis, were associated with a less chance of natural infection in the studied dogs. Prevention and control of the enzootic transmission risk of TG Rickettsia should help to reduce the potential zoonotic transmission of this pathogen.

Molecular confirmation of Rickettsia parkeri in humans from Southern Mexico.

Human tick-borne rickettsioses are caused by Rickettsia africae, Rickettsia massiliae, Rickettsia parkeri, Rickettsia philipi and Rickettsia rickettsii in the American continent. Rickettsia parkeri represents the second most important agent of eschar-associated spotted fever throughout the American continent. In Mexico, the presence of three strains of this bacterium (Black Gap, Maculatum and Atlantic Rainforest) has recently been reported in four species of ticks from five states (Baja California, Campeche, Sonora, Tabasco and Veracruz) and rickettsaemia was demonstrated in a dog from the state of Yucatán. We report two human cases of R. parkeri-rickettsiosis from Yucatan, Mexico. These findings have increased the interest in R. parkeri surveillance in the human population, due to the fluctuating regional Spotted Fever morbidity and mortality in the country.

Natural infection with Trypanosoma cruzi in bats captured in Campeche and Yucatán, México. / Infección natural con Trypanosoma cruzi en murciélagos capturados en Campeche y Yucatán, México

INTRODUCTION: Bats have been reported as hosts of the Trypanosoma cruzi protozoan, the etiologic agent of American trypanosomiasis, an endemic zoonotic disease in México. OBJECTIVE: To describe T. cruzi infection in bats from the states of Campeche and Yucatán, México. MATERIALS AND METHODS: Captures were made from March to November, 2017, at three sites in Yucatán and one in Campeche. Up to four mist nets on two consecutive nights were used for the capture. The bats' species were identified and euthanasia was performed to collect kidney and heart samples for total DNA extraction. Trypanosoma cruzi infection was detected by conventional PCR with the amplification of a fragment belonging to the T. cruzi DNA nuclear. RESULTS: Eighty-six bats belonging to five families (Vespertilionidae, Noctilionidae, Mormoopidae, Phyllostomidae, and Molossidae) and 13 species (Rhogeessa aeneus, Noctilio leporinus, Pteronotus davyi, P. parnellii, Artibeus jamaicensis, A. lituratus, A. phaeotis, Glossophaga soricina, Carollia sowelli, Chiroderma villosum, Uroderma bilobatum, Sturnira parvidens, and Molossus rufus) were captured. Infection frequency by PCR was 30,2% (26/86) detected only in the renal tissue. The infected species were P. parnellii, G. soricina, A. lituratus, A. jamaicensis, S. parvidens, C. villosum, and R. aeneus. CONCLUSIONS: Our results confirmed the participation of several bat species as hosts in the T. cruzi transmission cycle in the region. Further studies are necessary to establish the importance of these animals in the zoonotic transmission of T. cruzi.

Introducción. Los murciélagos se han reportado como huéspedes del protozoario Trypanosoma cruzi, agente etiológico de la tripanosomiasis americana, enfermedad zoonótica endémica en México. Objetivo. Describir la infección con T. cruzi en murciélagos capturados en los estados de Campeche y Yucatán, México. Materiales y métodos. Se realizaron capturas de marzo a noviembre de 2017 en tres sitios de Yucatán y uno de Campeche. Para la captura se emplearon hasta cuatro redes de niebla por dos noches consecutivas. Se identificó la especie de los murciélagos capturados y se les practicó la eutanasia para recolectar muestras de riñón y corazón, utilizadas posteriormente en la extracción de ADN total. La infección con T. cruzi se detectó por la amplificación con PCR convencional de un fragmento perteneciente al ADN nuclear de T. cruzi. Resultados. Se capturaron 86 murciélagos pertenecientes a cinco familias (Vespertilionidae, Noctilionidae, Mormoopidae, Phyllostomidae, Molossidae) y 13 especies (Rhogeessa aeneus, Noctilio leporinus, Pteronotus davyi, P. parnellii, Artibeus jamaicensis, A. lituratus, A. phaeotis, Glossophaga soricina, Carollia sowelli, Chiroderma villosum, Uroderma bilobatum, Sturnira parvidens y Molossus rufus). La PCR mostró una frecuencia de infección de 30,2 % (26/86), detectada únicamente en tejido renal. Las especies infectadas fueron P. parnellii, G. soricina, A. lituratus, A. jamaicensis, S. parvidens, C. villosum y R. aeneus. Conclusiones. Los resultados confirmaron la participación de varias especies de murciélagos como huéspedes en el ciclo de transmisión de T. cruzi en la región. Es necesario realizar más estudios para determinar la importancia de estos animales en la transmisión zoonótica de T. cruzi.

Natural infection with Trypanosoma cruzi in bats captured in Campeche and Yucatán, México / Infección natural con Trypanosoma cruzi en murciélagos capturados en Campeche y Yucatán, México

Abstract | Introduction: Bats have been reported as hosts of the Trypanosoma cruzi protozoan, the etiologic agent of American trypanosomiasis, an endemic zoonotic disease in México. Objective: To describe T. cruzi infection in bats from the states of Campeche and Yucatán, México. Materials and methods: Captures were made from March to November, 2017 at three sites in Yucatán and one in Campeche. Up to four mist nets on two consecutive nights were used for the capture. The bats' species were identified and euthanasia was performed to collect kidney and heart samples for total DNA extraction. Trypanosoma cruzi infection was detected by conventional PCR with the amplification of a fragment belonging to the T. cruzi DNA nuclear. Results: Eighty-six bats belonging to five families (Vespertilionidae, Noctilionidae, Mormoopidae, Phyllostomidae, and Molossidae) and 13 species (Rhogeessa aeneus, Noctilio leporinus, Pteronotus davyi, P. parnellii, Artibeus jamaicensis, A. lituratus, A. phaeotis, Glossophaga soricina, Carollia sowelli, Chiroderma villosum, Uroderma bilobatum, Sturnira parvidens, and Molossus rufus) were captured. Infection frequency by PCR was 30,2% (26/86) detected only in the renal tissue. The infected species were P. parnellii, G. soricina, A. lituratus, A. jamaicensis, S. parvidens, C. villosum, and R. aeneus. Conclusions: Our results confirmed the participation of several bat species as hosts in the T. cruzi transmission cycle in the region. Further studies are necessary to establish the importance of these animals in the zoonotic transmission of T. cruzi.

Resumen | Introducción. Los murciélagos se han reportado como huéspedes del protozoario Trypanosoma cruzi, agente etiológico de la tripanosomiasis americana, enfermedad zoonótica endémica en México. Objetivo. Describir la infección con T. cruzi en murciélagos capturados en los estados de Campeche y Yucatán, México. Materiales y métodos. Se realizaron capturas de marzo a noviembre de 2017 en tres sitios de Yucatán y uno de Campeche. Para la captura se emplearon hasta cuatro redes de niebla por dos noches consecutivas. Se identificó la especie de los murciélagos capturados y se les practicó la eutanasia para recolectar muestras de riñón y corazón, utilizadas posteriormente en la extracción de ADN total. La infección con T. cruzi se detectó por la amplificación con PCR convencional de un fragmento perteneciente al ADN nuclear de T. cruzi. Resultados. Se capturaron 86 murciélagos pertenecientes a cinco familias (Vespertilionidae, Noctilionidae, Mormoopidae, Phyllostomidae, Molossidae) y 13 especies (Rhogeessa aeneus, Noctilio leporinus, Pteronotus davyi, P. parnellii, Artibeus jamaicensis, A. lituratus, A. phaeotis, Glossophaga soricina, Carollia sowelli, Chiroderma villosum, Uroderma bilobatum, Sturnira parvidens y Molossus rufus). La PCR mostró una frecuencia de infección de 30,2 % (26/86), detectada únicamente en tejido renal. Las especies infectadas fueron P. parnellii, G. soricina, A. lituratus, A. jamaicensis, S. parvidens, C. villosum y R. aeneus. Conclusiones. Los resultados confirmaron la participación de varias especies de murciélagos como huéspedes en el ciclo de transmisión de T. cruzi en la región. Es necesario realizar más estudios para determinar la importancia de estos animales en la transmisión zoonótica de T. cruzi.

West Nile and Zika viruses in bats from a suburban area of Merida, Yucatan, Mexico.

Infections with viruses of the Flavivirus genus were explored in 22 bats (Artibeus jamaicensis) from Merida, Yucatan, Mexico. The detection of the viral genus was performed by RT-PCR, and infections with dengue (DENV 1-4), West Nile (WNV) and Zika (ZIKV) viruses were subsequently explored. Sequences from positive products were analysed using the BLAST algorithm to determine identity. In 7 (31.8%) and 2 (9.1%) bats, WNV and ZIKV were identified, respectively. The bioinformatic analysis showed 98%-100% coverage and identity for both viruses. Molecular evidence of WNV and ZIKV natural infection in bats from Yucatan, Mexico, is presented.

Leptospira patógena en murciélagos de Campeche y Yucatán, México / Pathogenic Leptospira in bats from Campeche and Yucatán, Mexico

RESUMEN Objetivo. Reportar la infección con Leptospira en ríñones de murciélagos de Campeche y Yucatán, México, a través de la amplificación por PCR de dos fragmentos distintos del gen 16S RNA ribosomal. Materiales y métodos. Se realizaron capturas en un sitio de Campeche y dos de Yucatán. A los murciélagos capturados se les aplicó la eutanasia y se les realizó una necropsia para recolectar tejido renal que se usó en la extracción de ADN total. Se realizaron dos PCR convencionales para la amplificación de los fragmentos de 16S RNA ribosomal. Se obtuvieron las secuencias de algunos productos positivos y se analizaron con herramientas bioinformáticas para identificar la especie infectante de Leptospira. Resultados. Se capturaron 69 murciélagos pertenecientes a cuatro familias y a ocho especies distintas. La familia con mayor diversidad fue Phyllostomidae con cinco especies. La especie con mayor frecuencia de captura fue Artibeusjamaicensis (41, 59.4%). Las PCR arrojaron una frecuencia global de infección de 21.7%. Las especies infectadas fueron A. jamaicensis, Pteronotus parnellii y Chiroderma villosum. El análisis bioinformático arrojó un 99.0% de identidad para Leptospira noguchii, Leptospira borgpetersenii y Leptospira santarosai. Conclusiones. Algunas especies de murciélagos de Yucatán y Campeche son portadores renales de leptospiras patógenas, por lo que podrían participar en el ciclo silvestre de transmisión en la región. La frecuencia de infección encontrada en los riñones de los murciélagos utilizados es mayor en comparación con aquellas obtenidas en otros reservorios de Yucatán y Campeche. Nuevas especies de murciélagos son reportadas como portadores de Leptospira para México.

ABSTRACT Objective. To report the infection with Leptospira in the kidneys of bats from Campeche and Yucatán, Mexico, through the amplification by PCR of two different 16S RNA ribosomal gene fragments. Materials and methods. Bat captures were carried out at one site in Campeche and two sites in Yucatán. Euthanasia was applied to the captured bats and a necropsy was performed to collect a renal tissue sample that was used in the total DNA extraction. Two different conventional PCR were performed for the amplification of the 16S RNA ribosomal gene fragments. Some sequences from positive products were obtained and analyzed with bioinformatics tools to identify the infectious species of Leptospira. Results. Sixty-nine bats belonging to four families and eight different species were captured. The family with the greatest diversity was Phyllostomidae with five species. The most captured species was Artibeus jamaicensis (41, 59.4%). Both PCR showed a global infection frequency of 21.7%. The infected species were A. jamaicensis, Pteronotus parnellii, and Chiroderma villosum. The bioinformatic analysis of the positive products yielded a 99.0% identity for Leptospira noguchii, Leptospira borgpetersenii, and Leptospira santarosai. Conclusions. Some bat species of Yucatán and Campeche, Mexico, are renal carriers of pathogenic Leptospira, therefore participating in the transmission cycle in the region. The frequency of infection found in the renal tissue of the captured bats is higher than the one obtained from other reservoirs captured in Yucatán and Campeche. New species of bats are reported as renal Leptospira carriers in Mexico.

Personal and household factors involved in recent Rickettsia exposure in a rural population from Yucatán, Mexico.

The aim of the study was to describe the epidemiological factors associated with the risks of recent Rickettsia exposure in inhabitants of a rural population from Yucatán, Mexico. The study included 130 inhabitants from Maxcanú, Yucatán. Blood samples were collected to detect IgM and IgG antibodies against Rickettsia typhi and Rickettsia rickettsii by an indirect immunofluorescence antibody test. Additionally, nested polymerase chain reaction was performed to amplify fragments of the 17kDa and sca5 genes. Previously, an epidemiological questionnaire was applied focused on collecting information on personal and housing exposure variables related to the recent infection with Rickettsia to determine epidemiological associations. Results that exhibited a p-value < .25 were included in a generalized multinomial logistic linear model to determine the variables involved with the risk of contact or Rickettsia infection. In all, 76% (99/130) of the participants presented with immunoglobulin titres against the Rickettsia species evaluated, while rickettsial DNA was detected in 35.4% (46/130) of the participants. The association analysis with the personal exposure variables showed that the productive age group (OR = 0.32; 95% CI = 0.10-1.03) and the elders group (OR = 0.12; 95% CI = 0.01-0.83) were protective factors for recent infection with R. typhi, taking as reference the school group. The presence of a family orchard in the home (OR = 7.56; 95% CI = 1.62-35.29) was a risk factor for recent infection with R. rickettsii. Presumably, the presence of ectoparasites (OR = 2.71; 95% CI = 0.90-8.09) at home was a risk factor for recent infection with both Rickettsia species. Recent infection was demonstrated in inhabitants from Maxcanú, Yucatán. A high seropositive frequency was obtained. The results highlight the importance of the family garden and the presence of ectoparasites in the home as risk factors associated with recent infection with Rickettsia in inhabitants from Maxcanú.

Infección con Toxoplasma gondii (Eucoccidiorida: Sarcocystidae) en murciélagos de Campeche y Yucatán, México

Toxoplasma gondii es un protozoario parásito reconocido como el agente causal de la toxoplasmosis, enfermedad zoonótica que afecta a humanos y animales domésticos o silvestres. En México, representa un problema de salud pública y veterinaria, sobre todo en regiones con climas tropicales y subtropicales. Los murciélagos han sido identificados como hospederos accidentales en el ciclo de transmisión; no obstante, en México no existe información previa; por lo tanto, el objetivo del presente estudio es reportar la infección con T. gondii en murciélagos capturados en sitios de los estados de Campeche y Yucatán, México. Se capturaron murciélagos en dos sitios de Yucatán y uno de Campeche, ubicados en la Península de Yucatán. Se recolectaron riñones, bazo e hígado y se emplearon en la extracción de ADN total. La infección con T. gondii se detectó a través de la amplificación de un fragmento del gen B1, utilizando PCR anidada. Los productos positivos fueron purificados y enviados a secuenciación para su posterior análisis de alineamiento; adicionalmente, se construyó un árbol filogenético. Se analizaron un total de 69 murciélagos pertenecientes a ocho especies distintas: 41 (59.4 %, 41/69) Artibeus jamaicensis; seis (8.7 %, 6/69) Pteronotus parnellii; seis (8.7 %, 6/69) Noctilio leporinus; seis (8.7 %, 6/69) Chiroderma villosum; cuatro (5.8 %, 4/69) Glossophaga soricina; dos (2.9 %, 2/69) Carollia sowelli; dos (2.89 %, 2/69) Artibeus lituratus y dos (2.9%, 2/69) Rhogeessa aeneus. La PCR anidada identificó ocho (11.6 %, 8/69) murciélagos positivos a la infección: seis (75 %, 6/8) A. jamaicensis, capturados en X'matkuil y Panabá, un (12.5 %, 1/8) G. soricina y un (12.5 %, 1/8) C. villosum, ambos capturados en Panabá. El análisis de alineamiento arrojó 99-100 % para cobertura y 97-99 % para identidad respecto a secuencias de T. gondii. Nuestros resultados aportan al entendimiento del ciclo de transmisión de T. gondii en la región; sin embargo, son necesarias investigaciones futuras para determinar los genotipos circulantes, ya que estudios anteriores han demostrado que estos animales pueden estar infectados con genotipos identificados en otros animales domésticos o silvestres e incluso en humanos.

Toxoplasma gondii is a protozoan parasite recognized as the causative agent of toxoplasmosis, a zoonotic disease that affects humans and domestic or wild animals. In Mexico, it represents a public and animal health problem, especially in regions with tropical and subtropical climates. Bats have been reported as accidental hosts in the transmission cycle; however, there is no preceding information in Mexico. Therefore, the aim of the present study is to report the T. gondii infection in bats captured in sites of Campeche and Yucatan states, Mexico. Bats were captured in two sites in Yucatan (X'matkuil and Panaba) and one in Campeche (Hampolol), located in the Yucatan Peninsula. Kidneys, spleen, and liver were collected and used in the total DNA extraction. Toxoplasma gondii infection was detected through the amplification of a B1 gene fragment, using nested PCR. The positive PCR products were purified and sent to sequencing for a posterior sequence identity analysis. Additionally, a phylogenetic tree was made. A total of 69 bats belonging to eight different species were processed: 41 (59.4 %, 41/69) Artibeus jamaicensis; six (8.7 %, 6/69) Pteronotus parnellii; six (8.7 %, 6/69) Noctilio leporinus; six (8.7 %, 6/69) Chiroderma villosum; four (5.8 %, 4/69) Glossophaga soricina; two (2.9 %, 2/69) Carollia sowelli; two (2.89 %, 2/69) Artibeus lituratus; and two (2.9 %, 2/69) Rhogeessa aeneus. The nested PCR identified eight (11.6 %, 8/69) infected bats: six (75 %, 6/8) A. jamaicensis, captured in X'matkuil and Panaba, one (12.5 %, 1/8) G. soricina, and one (12.5 %, 1/8) C. villosum, both captured in Panaba. The alignment analysis yielded 99-100 % for cover and 97-99 % for identity to T. gondii sequences. Our results contribute to the understanding of the T. gondii transmission cycle in the region; however, future research is needed to determine circulating genotypes, as previous studies have demonstrated that these animals might be infected with identified genotypes in other domestic or wild animals and even in humans.

Detección molecular de leptospiras patógenas en roedores sinantrópicos y silvestres capturados en Yucatán, México / Molecular detection of pathogenic Leptospira in synanthropic and wild rodents captured in Yucatán, México

Introducción. La leptospirosis es una enfermedad zoonótica endémica en México, ocasionada por la bacteria del género Leptospira, la cual constituye un problema de salud pública y veterinaria. Los roedores son los reservorios más relevantes de Leptospira spp., debido a que la bacteria se establece y se reproduce en su tejido renal y es excretada por la orina. Objetivo. Identificar la presencia de Leptospira spp. en tejido renal de roedores capturados en Yucatán, México. Materiales y métodos. Se capturaron roedores sinantrópicos y silvestres en el municipio rural de Cenotillo, Yucatán, México. Se tomó un riñón de cada roedor y se extrajo el ADN total. La identificación de Leptospira spp. se hizo mediante la detección de dos fragmentos del gen 16S rRNA con una reacción en cadena de la polimerasa (PCR) de punto final. Los productos positivos se secuenciaron y se analizaron con herramientas de alineamiento. Resultados. Se capturaron 92 roedores pertenecientes a siete especies distintas. La PCR arrojó 5,4 % (5/92) de positividad global. El análisis del alineamiento de los aislamientos de los roedores infectados demostró 100 % de cobertura e identidad con la especie Leptospira interrogans. Esta es la primera evidencia molecular de la circulación de Leptospira spp. en Heteromys gaumeri capturados en Yucatán, México. Conclusión. Se evidenció que los roedores de Yucatán, México, son reservorios de Leptospira spp. y participan en el ciclo de infección de la leptospirosis en la región.

Introduction: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which is endemic in México and considered a public and veterinary health problem. Rodents are the most relevant reservoirs of Leptospira spp. because the bacteria establish and reproduce in its renal tissue and are excreted through the urine. Objective: To identify the presence of Leptospira spp. in renal tissue from rodents captured in Yucatán, México. Materials and methods: Synanthropic and wild rodents were captured in the rural municipality of Cenotillo, Yucatán, México. We collected one kidney from each rodent and extracted the total DNA. The identification of Leptospira spp. was done by detecting two fragments of the 16S rRNA gene using end-point polymerase chain reaction (PCR). We sequenced and analyzed positive products using alignment tools. Results: A total of 92 rodents belonging to seven different species were captured. The PCR yielded a global positivity of 5.4% (5/92). The alignment analysis of the sequenced products demonstrated a 100% of coverage and identity with Leptospira interrogans. This is the first molecular evidence of Leptospira spp. circulation in Heteromys gaumeri captured in Yucatán, México. Conclusion: Our results evidenced that rodents of Yucatán are reservoirs of Leptospira spp. and participate in the infection cycle of leptospirosis in the region.

Detection of hand, foot and mouth disease in the yucatan peninsula of Mexico.

We report a case of hand, foot and mouth disease (HFMD) in a 5-year-old male from Merida City in the Yucatan Peninsula of Mexico. A clinical and physical examination revealed that the patient had symptoms typical of HFMD, including fever, fatigue, odynophagia, throat edema, hyperemia, lesions on the hands and feet, and blisters in the oral cavity. The patient fully recovered after a convalescence period of almost three weeks. Reverse transcription-polymerase chain reaction and nucleotide sequencing revealed that the etiological agent was enterovirus 71 (EV71). The sequence has greatest (90.4%) nucleotide identity to the corresponding regions of EV71 isolates from the Netherlands and Singapore. Although HFMD is presumably common in Mexico, surprisingly there are no data in the PubMed database to support this. This case report provides the first peer-reviewed evidence of HFMD in Mexico.